Microtiter assay for interferon: microspectrophotometric quantitation of cytopathic effect

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Microtiter assay for interferon: microspectrophotometric quantitation of cytopathic effect.

This report describes an accurate, reproducible, and efficient microassay for human interferon, using a dye-uptake method to quantitate cytopathogenicity. The antiviral activity was measured by using a Gilford 300-N microsepctrophotometer, with the automatic programmer, sampler, and data lister. Two hundred interferon samples, each in a final volume of 1.0 ml, may be analyzed and recorded in 45...

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A rapid, cytopathic effect reduction assay for human interferon (IFN) is described. Dilutions of IFN were made with an automated diluter in 96-well microtiter plates. Total incubation time was 26 h. IFN titers were calculated from optical density readings of crystal violet-stained monolayers in an automated spectrophotometer, which required less than 1 min to read each plate.

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1976

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.31.1.35-38.1976